Recent economic reforms and political changes within Southern Africa have opened up new opportunities for intra-regional agricultural trade and other forms of agribusiness collaboration. This collaboration is vital, given the relatively small size of individual country markets and the fact that no single country in the region has the resources and capacity to mount a sustained drive to achieve international market prominence and competitiveness. This study examines private sector perceptions regarding the agribusiness investment environment and the scope for regional collaboration in Southern Africa. It then presents a series of case studies highlighting experiences in such collaboration as well as additional opportunities and constraints. The case studies illustrate the varied forms that this intra-regional collaboration has or could take, including the transfer of technologies and management systems, joint logistics and international marketing, cross-border investment, product research and development, human resource development, raw material sourcing, and regional trade in inputs and consumer products. Both the agribusiness company survey and the industry case studies include recommendations for policymakers, agribusiness managers/representatives, and agricultural and private sector development practitioners.