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Publisher's Note: This manual will give students a chance to explore the process and techniques of characterizing and purifying a protein and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis. A summary of relevant techniques supplements actual laboratory exercises. Topics and techniques are chosen based on input from active researchers in the biotechnology/pharmaceutical sciences. This book is designed for students of biotechnology and is suitable both as a course manual for academic and professional education programs, and for self-study and review. Tried and tested experiments from each area are assembled around a common theme. They not only teach valuable skills, but also demonstrate the research process used in biotechnology laboratories.